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rosettesep human nk cell enrichment antibody cocktail (STEMCELL Technologies Inc)

Name: rosettesep human nk cell enrichment antibody cocktail
Brand: STEMCELL Technologies Inc
Rating: 90 (1 reviews)

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STEMCELL Technologies Inc rosettesep human nk cell enrichment antibody cocktail

Effects of TC exposures on the ability of <t>NK</t> <t>cells</t> to lyse tumor <t>cells.</t> <t>NK</t> cells were exposed to 0.5-10 μM TC for 1 hr, 24 hr, 48 hr, or 6 d. To combine results from separate experiments (using cells from different donors) the levels of lysis were normalized as the percentage of the lytic function of the control cells in a given experiment. Results were from three separate experiments using different donors (triplicate determinations for each experiment; n = 9, mean ± SD). *Statistically significant change as compared to control cells at that same length of incubation (p < 0.05).

Rosettesep Human Nk Cell Enrichment Antibody Cocktail, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rosettesep human nk cell enrichment antibody cocktail/product/STEMCELL Technologies Inc
Average 90 stars, based on 1 article reviews

rosettesep human nk cell enrichment antibody cocktail - by Bioz Stars, 2026-03

90/100 stars

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1) Product Images from "Immunosuppressive Effects of Triclosan, Nonylphenol, and DDT on Human Natural Killer Cells In Vitro"

Article Title: Immunosuppressive Effects of Triclosan, Nonylphenol, and DDT on Human Natural Killer Cells In Vitro

Journal:

doi: 10.3109/15476911003667470

Effects of TC exposures on the ability of NK cells to lyse tumor cells. NK cells were exposed to 0.5-10 μM TC for 1 hr, 24 hr, 48 hr, or 6 d. To combine results from separate experiments (using cells from different donors) the levels of lysis were normalized as the percentage of the lytic function of the control cells in a given experiment. Results were from three separate experiments using different donors (triplicate determinations for each experiment; n = 9, mean ± SD). *Statistically significant change as compared to control cells at that same length of incubation (p < 0.05).

Figure Legend Snippet: Effects of TC exposures on the ability of NK cells to lyse tumor cells. NK cells were exposed to 0.5-10 μM TC for 1 hr, 24 hr, 48 hr, or 6 d. To combine results from separate experiments (using cells from different donors) the levels of lysis were normalized as the percentage of the lytic function of the control cells in a given experiment. Results were from three separate experiments using different donors (triplicate determinations for each experiment; n = 9, mean ± SD). *Statistically significant change as compared to control cells at that same length of incubation (p < 0.05).

Techniques Used: Lysis, Incubation

Effects of 1 hr exposures to TC followed by 24 hr, 48 hr, or 6 d in TC-free media on the ability of NK cells to lyse tumor cells. NK cells were exposed to 2.5–10 μM TC for 1 hr. Results were from at least three separate experiments using different donors (triplicate determinations for each experiment; n = 9, mean ± SD, as described in Figure 1. *Statistically significant change as compared to control cells at that same length of incubation (p < 0.05).

Figure Legend Snippet: Effects of 1 hr exposures to TC followed by 24 hr, 48 hr, or 6 d in TC-free media on the ability of NK cells to lyse tumor cells. NK cells were exposed to 2.5–10 μM TC for 1 hr. Results were from at least three separate experiments using different donors (triplicate determinations for each experiment; n = 9, mean ± SD, as described in Figure 1. *Statistically significant change as compared to control cells at that same length of incubation (p < 0.05).

Techniques Used: Incubation

Effects of NP exposures on the ability of NK cells to lyse tumor cells. NK cells were exposed to 1-10 μM NP for 1 hr, 24 hr, 48 hr, or 6 d. Results were from three separate experiments using different donors (triplicate determinations for each experiment, n = 9, mean ± SD), as described in Figure 1. *Statistically significant change as compared to control cells at that same length of incubation (p < 0.05).

Figure Legend Snippet: Effects of NP exposures on the ability of NK cells to lyse tumor cells. NK cells were exposed to 1-10 μM NP for 1 hr, 24 hr, 48 hr, or 6 d. Results were from three separate experiments using different donors (triplicate determinations for each experiment, n = 9, mean ± SD), as described in Figure 1. *Statistically significant change as compared to control cells at that same length of incubation (p < 0.05).

Techniques Used: Incubation

Effects of 1 hr exposures to NP followed by 24 hr, 48 hr, or 6 d in NP-free media on the ability of NK cells to lyse tumor cells. NK cells were exposed to 2.5–10 μM NP for 1 hr. Results were from at least three separate experiments using different donors (triplicate determinations for each experiment; n = 9, mean ± SD), as described in Figure 1. *Statistically significant change as compared to control cells at that same length of incubation (p < 0.05).

Figure Legend Snippet: Effects of 1 hr exposures to NP followed by 24 hr, 48 hr, or 6 d in NP-free media on the ability of NK cells to lyse tumor cells. NK cells were exposed to 2.5–10 μM NP for 1 hr. Results were from at least three separate experiments using different donors (triplicate determinations for each experiment; n = 9, mean ± SD), as described in Figure 1. *Statistically significant change as compared to control cells at that same length of incubation (p < 0.05).

Techniques Used: Incubation

Effects of DDT exposures on the ability of NK cells to lyse tumor cells. NK cells were exposed to 0.25-10 μM DDT for 1 hr, 24 hr, 48 hr, or 6 d. Results were from three separate experiments using different donors (triplicate determinations for each experiment; n = 9, mean ± SD), as described in Figure 1. *Statistically significant change as compared to control cells at that same length of incubation (p < 0.05).

Figure Legend Snippet: Effects of DDT exposures on the ability of NK cells to lyse tumor cells. NK cells were exposed to 0.25-10 μM DDT for 1 hr, 24 hr, 48 hr, or 6 d. Results were from three separate experiments using different donors (triplicate determinations for each experiment; n = 9, mean ± SD), as described in Figure 1. *Statistically significant change as compared to control cells at that same length of incubation (p < 0.05).

Techniques Used: Incubation

Effects of 1 hr exposures to DDT followed by 24 hr, 48 hr, or 6 d in DDT-free media on the ability of NK cells to lyse tumor cells. NK cells were exposed to 1–5 μM DDT for 1 hr. Results were from at least three separate experiments using different donors (triplicate determinations for each experiment; n = 9, mean ± SD), as described in Figure 1. *Statistically significant change as compared to control cells at that same length of incubation (p < 0.05).

Figure Legend Snippet: Effects of 1 hr exposures to DDT followed by 24 hr, 48 hr, or 6 d in DDT-free media on the ability of NK cells to lyse tumor cells. NK cells were exposed to 1–5 μM DDT for 1 hr. Results were from at least three separate experiments using different donors (triplicate determinations for each experiment; n = 9, mean ± SD), as described in Figure 1. *Statistically significant change as compared to control cells at that same length of incubation (p < 0.05).

Techniques Used: Incubation

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Journal:

Article Title: Immunosuppressive Effects of Triclosan, Nonylphenol, and DDT on Human Natural Killer Cells In Vitro

doi: 10.3109/15476911003667470

Figure Lengend Snippet: Effects of TC exposures on the ability of NK cells to lyse tumor cells. NK cells were exposed to 0.5-10 μM TC for 1 hr, 24 hr, 48 hr, or 6 d. To combine results from separate experiments (using cells from different donors) the levels of lysis were normalized as the percentage of the lytic function of the control cells in a given experiment. Results were from three separate experiments using different donors (triplicate determinations for each experiment; n = 9, mean ± SD). *Statistically significant change as compared to control cells at that same length of incubation (p < 0.05).

Article Snippet: Buffy coats were mixed with 0.8 ml of RosetteSep human NK cell enrichment antibody cocktail (StemCell Technologies, Vancouver, British Columbia, Canada) per 45 ml of buffy coat.

Techniques: Lysis, Incubation

Journal:

Article Title: Immunosuppressive Effects of Triclosan, Nonylphenol, and DDT on Human Natural Killer Cells In Vitro

doi: 10.3109/15476911003667470

Figure Lengend Snippet: Effects of 1 hr exposures to TC followed by 24 hr, 48 hr, or 6 d in TC-free media on the ability of NK cells to lyse tumor cells. NK cells were exposed to 2.5–10 μM TC for 1 hr. Results were from at least three separate experiments using different donors (triplicate determinations for each experiment; n = 9, mean ± SD, as described in Figure 1. *Statistically significant change as compared to control cells at that same length of incubation (p < 0.05).

Article Snippet: Buffy coats were mixed with 0.8 ml of RosetteSep human NK cell enrichment antibody cocktail (StemCell Technologies, Vancouver, British Columbia, Canada) per 45 ml of buffy coat.

Techniques: Incubation

Journal:

Article Title: Immunosuppressive Effects of Triclosan, Nonylphenol, and DDT on Human Natural Killer Cells In Vitro

doi: 10.3109/15476911003667470

Figure Lengend Snippet: Effects of NP exposures on the ability of NK cells to lyse tumor cells. NK cells were exposed to 1-10 μM NP for 1 hr, 24 hr, 48 hr, or 6 d. Results were from three separate experiments using different donors (triplicate determinations for each experiment, n = 9, mean ± SD), as described in Figure 1. *Statistically significant change as compared to control cells at that same length of incubation (p < 0.05).

Article Snippet: Buffy coats were mixed with 0.8 ml of RosetteSep human NK cell enrichment antibody cocktail (StemCell Technologies, Vancouver, British Columbia, Canada) per 45 ml of buffy coat.

Techniques: Incubation

Journal:

Article Title: Immunosuppressive Effects of Triclosan, Nonylphenol, and DDT on Human Natural Killer Cells In Vitro

doi: 10.3109/15476911003667470

Figure Lengend Snippet: Effects of 1 hr exposures to NP followed by 24 hr, 48 hr, or 6 d in NP-free media on the ability of NK cells to lyse tumor cells. NK cells were exposed to 2.5–10 μM NP for 1 hr. Results were from at least three separate experiments using different donors (triplicate determinations for each experiment; n = 9, mean ± SD), as described in Figure 1. *Statistically significant change as compared to control cells at that same length of incubation (p < 0.05).

Article Snippet: Buffy coats were mixed with 0.8 ml of RosetteSep human NK cell enrichment antibody cocktail (StemCell Technologies, Vancouver, British Columbia, Canada) per 45 ml of buffy coat.

Techniques: Incubation

Journal:

Article Title: Immunosuppressive Effects of Triclosan, Nonylphenol, and DDT on Human Natural Killer Cells In Vitro

doi: 10.3109/15476911003667470

Figure Lengend Snippet: Effects of DDT exposures on the ability of NK cells to lyse tumor cells. NK cells were exposed to 0.25-10 μM DDT for 1 hr, 24 hr, 48 hr, or 6 d. Results were from three separate experiments using different donors (triplicate determinations for each experiment; n = 9, mean ± SD), as described in Figure 1. *Statistically significant change as compared to control cells at that same length of incubation (p < 0.05).

Article Snippet: Buffy coats were mixed with 0.8 ml of RosetteSep human NK cell enrichment antibody cocktail (StemCell Technologies, Vancouver, British Columbia, Canada) per 45 ml of buffy coat.

Techniques: Incubation

Journal:

Article Title: Immunosuppressive Effects of Triclosan, Nonylphenol, and DDT on Human Natural Killer Cells In Vitro

doi: 10.3109/15476911003667470

Figure Lengend Snippet: Effects of 1 hr exposures to DDT followed by 24 hr, 48 hr, or 6 d in DDT-free media on the ability of NK cells to lyse tumor cells. NK cells were exposed to 1–5 μM DDT for 1 hr. Results were from at least three separate experiments using different donors (triplicate determinations for each experiment; n = 9, mean ± SD), as described in Figure 1. *Statistically significant change as compared to control cells at that same length of incubation (p < 0.05).

Article Snippet: Buffy coats were mixed with 0.8 ml of RosetteSep human NK cell enrichment antibody cocktail (StemCell Technologies, Vancouver, British Columbia, Canada) per 45 ml of buffy coat.

Techniques: Incubation

Effects of 24 h, 48 h and 6 day exposures to TBT on IL-6 secretion from highly purified human NK cells, monocyte-depleted PBMCs, PBMCs, PBMCs plus granulocytes, and granulocytes in individual donors. A) NK cells exposed to 0–200 nM TBT (donor KB169). B) Monocyte-depleted PBMCs exposed to 0–200 nM TBT (donor F156). C) PBMCs exposed to 0–200 nM TBT (donor F261). D) PBMCs plus granulocytes exposed to 0–200 nM TBT (donor F261). E) Granulocytes exposed to 0–200 nM TBT (donor F261). * Indicates a significant decrease in secretion and + indicates a significant increase in secretion compared to control cells (cells treated with vehicle alone).

Journal: Journal of applied toxicology : JAT

Article Title: BUTYLTIN COMPOUNDS ALTER SECRETION OF INTERLEUKIN 6 FROM HUMAN IMMUNE CELLS

doi: 10.1002/jat.3514

Figure Lengend Snippet: Effects of 24 h, 48 h and 6 day exposures to TBT on IL-6 secretion from highly purified human NK cells, monocyte-depleted PBMCs, PBMCs, PBMCs plus granulocytes, and granulocytes in individual donors. A) NK cells exposed to 0–200 nM TBT (donor KB169). B) Monocyte-depleted PBMCs exposed to 0–200 nM TBT (donor F156). C) PBMCs exposed to 0–200 nM TBT (donor F261). D) PBMCs plus granulocytes exposed to 0–200 nM TBT (donor F261). E) Granulocytes exposed to 0–200 nM TBT (donor F261). * Indicates a significant decrease in secretion and + indicates a significant increase in secretion compared to control cells (cells treated with vehicle alone).

Article Snippet: RosetteSep human NK cell enrichment antibody cocktail (0.6–0.8 mL) (StemCell Technologies, Vancouver, British Columbia, Canada) was added to 45 mL of buffy coat.

Techniques: Purification

Effects of 24 h, 48 h and 6 day exposures to DBT on IL-6 secretion from highly purified human NK cells, monocyte-depleted PBMCs, PBMCs, PBMCs plus granulocytes, and granulocytes in individual donors. A) NK cells exposed to 0–5 µM DBT (donor KB169). B) Monocyte-depleted PBMCs exposed to 0–5 µM DBT (donor F152). C) PBMCs exposed to 0–5 µM DBT (donor F285). D) PBMCs plus granulocytes exposed to 0–5 µM DBT (donor F285). E) Granulocytes exposed to 0–5 µM DBT (donor F285). * Indicates a significant decrease in secretion and + indicates a significant increase in secretion compared to control cells (cells treated with vehicle alone).

Journal: Journal of applied toxicology : JAT

Article Title: BUTYLTIN COMPOUNDS ALTER SECRETION OF INTERLEUKIN 6 FROM HUMAN IMMUNE CELLS

doi: 10.1002/jat.3514

Figure Lengend Snippet: Effects of 24 h, 48 h and 6 day exposures to DBT on IL-6 secretion from highly purified human NK cells, monocyte-depleted PBMCs, PBMCs, PBMCs plus granulocytes, and granulocytes in individual donors. A) NK cells exposed to 0–5 µM DBT (donor KB169). B) Monocyte-depleted PBMCs exposed to 0–5 µM DBT (donor F152). C) PBMCs exposed to 0–5 µM DBT (donor F285). D) PBMCs plus granulocytes exposed to 0–5 µM DBT (donor F285). E) Granulocytes exposed to 0–5 µM DBT (donor F285). * Indicates a significant decrease in secretion and + indicates a significant increase in secretion compared to control cells (cells treated with vehicle alone).

Article Snippet: RosetteSep human NK cell enrichment antibody cocktail (0.6–0.8 mL) (StemCell Technologies, Vancouver, British Columbia, Canada) was added to 45 mL of buffy coat.

Techniques: Purification

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1) Product Images from "Immunosuppressive Effects of Triclosan, Nonylphenol, and DDT on Human Natural Killer Cells In Vitro"

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